CREATION OF TISSUE-ENGINEERED LIVING BONE EQUIVALENT AND PROSPECTS FOR ITS APPLICATION IN TRAUMATOLOGY AND ORTHOPAEDICS

Objective. To present experimental prototypes of tissue-engineered bone equivalent (TEBE) based on nanostructured bioresorbable synthetic polymer cellular matrices (BSPCMs) and osteogenic differentiated cells created to repair bone defects.

Material and Methods. Nanostructured BSPCMs were developed and produced, which further served as the basis for creating TEBE. Cultured cells were transferred on the surface of nanostructured matrix, where patterns of their growth, expansion, and behavior were studied. Topography and surface properties of TEBE prototypes were investigated using methods of light-optical, scanning electron, and atomic force microscopy.

Results. A possibility of creating experimental TEBE prototype based on BSPCM, which copies to the maximum extent the bone structure at the micro- and nanoscales is shown. Surface of the BSPCM was additionally nanostructured by formation of longitudinally oriented nano-trenches, to increase adhesion and osteoconductive properties.

Conclusion. A strategy for creating nanostructured BSPCM and TEBE was developed, and experimental prototypes suitable for further investigations to form biodegradable implants for needs of traumatology, orthopaedics, and spine medicine were produced. 

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