ОСТЕОИНТЕГРАЦИЯ ГИДРОКСИАПАТИТОВЫХ ГРАНУЛ В ТЕЛАХ ПОЯСНИЧНЫХ ПОЗВОНКОВ В ЭКСПЕРИМЕНТЕ

Цель исследования. Верификация остеоинтеграции пористых биокерамических гранул на основе гидроксиапатита при гистологическом исследовании, включающем электронную сканирующую микроскопию, дополненную энергодисперсионной спектроскопией.

Материал и методы. Эксперимент проведен на 6 беспородных собаках весом от 15 до 18 кг, которым проводилась имплантация гидроксиапатитовых гранул в тела поясничных позвонков. Срок наблюдения – 6 мес., затем проводилось гистологическое исследование препаратов при помощи световой микроскопии, сканирующей электронной микроскопии и энергодисперсионной спектрометрии.

Результаты. При гистологическом исследовании зон имплантации гранул в пространстве между ними обнаружена сформированная костная ткань трабекулярного строения, которая плотно прилегала к их поверхности. На границе между костной тканью и гидроксиапатитовыми гранулами соединительно-тканная капсула отсутствовала.

Заключение. Морфологические методы исследования с применением световой микроскопии, а также методы сканирующей электронной микроскопии и энергодисперсионной спектрометрии являются базовыми для верификации остеоинтеграции.

1. Albrektsson T, Johansson C. Osteoinduction, osteoconduction and osseointegration. Eur Spine J. 2001; 10(Suppl 2): S96–S101.

2. Alexander D, Hoffmann J, Munz A, et al. Analysis of OPLA scaffolds for bone engineering constructs using human jaw periosteal cells. J Mater Sci Mater Med. 2008; 19: 965–974.

3. Banwart JC, Asher MA, Hassanein RS. Iliac crest bone graft harvest donor site morbidity. A statistical evaluation. Spine. 1995; 20: 1055–1060.

4. Becker S, Maissen O, Ponomarev I, et al. Osteopromotion by a beta‐tricalcium phosphate/bone marrow hybrid implant for use in spine surgery. Spine. 2006; 31: 11–17.

5. Ben‐Nissan B. Biomimetics and bioceramics. In: Reis RL, Weiner S (eds.). Learning from Nature How to Design New Implantable Biomaterialsis: From Biomineralization Fundamentals to Biomimetic Materials and Processing Routes. Netherlands: Kluwer Academic Publishers, 2004: 89–103.

6. Bose S, Darsell J, Hosick HL, et al. Processing and characterization of porous alumina scaffolds. J Mater Sci: Mater Med. 2002; 13: 23–28.

7. Branemark PI, Hansson BO, Adell R, et al. Osseointegrated implants in the treatment of the edentulous jaw. Experience from a 10-year period. Scand J Plast Reconstr Surg Suppl. 1977; 16: 1–132.

8. Branemark R, Branemark PI, Rydevik B, et al. Osseointegration in skeletal reconstruction and rehabilitation: a review. J Rehabil Res Dev. 2001; 38: 175–181.

9. Capanna R, De Biase P. Osteoinduction: Basic Principles and Developments. In: Leung KS, Tagland G, Schnetter R, et al. (eds). Practice of Intramedullary Locked Nails. Springer Berlin Heidelberg, 2006: 32–42.

10. Carter CB. Ceramics in biology and medicine. In: Carter CB, Norton MG (eds). Ceramic Materials: Science and Engineering. N. Y., 2007: 635–651. DOI: 10.1007/978-0-387-46271-4.

11. Daculsi G, Uzel AP, Weiss P, et al. Developments in injectable multiphasic biomaterials. The performance of microporous biphasic calcium phosphate granules and hydrogels. J Mater Sci Mater Med. 2009; 21: 855–861.

12. Damien CJ, Parsons JR. Bone graft and bone graft substitutes: a review of current technology and applications. J Appl Biomater. 1991; 2: 187–208.

13. Eroschenko VP. DiFiore’s Atlas of Histology with Functional Correlations, 11th ed. Lippincott Williams & Wilkins, 2008.

14. Green D, Walsh D, Mann S, et al. The potential of biomimesis in bone tissue engineering: lessons from the design and synthesis of invertebrate skeletons. Bone. 2002; 30: 810–815.

15. Lindgren C, Hallman M, Sennerby L, et al. Back-scattered electron imaging and elemental analysis of retrieved bone tissue following sinus augmentation with deproteinized bovine bone or biphasic calcium phosphate. Clin Oral Implants Res. 2010; 21: 924–930. DOI: 10.1111/j.1600-0501.2010.01933.x.

16. Nakamura T. Biomaterial osteoinduction. J Orthop Sci. 2007; 12: 111–112.

17. Nuss KM, von Rechenberg B. Biocompatibility issues with modern implants in bone – A review for clinical orthopedics. Open Orthop J. 2008; 2: 66–78.

18. Paderni S, Terzi S, Amendola L. Major bone defect treatment with an osteoconductive bone substitute. Chir Organi Mov. 2009; 93: 89–96.

19. Rerikh V, Avetisyan A, Aronov A, et al. In vivo implantation of new porous ceramic granules. Proceedings of 2nd International Conference on Competitive Materials and Technology Processes in Miskolc-Lillafüred, Hungary, October 8–12, 2012. Miskolc, 2012: 32.

20. Sandhu HS. Bone morphogenetic proteins and spinal surgery. Spine. 2003; 28(15 Suppl): S64–S73.

21. Shetty DC, Urs AB, Ahuja P, et al. Mineralized components and their interpretation in the histogenesis of peripheral ossifying fibroma. Indian J Dent Res. 2011; 22: 56–61.

22. Slater N, Dasmah A, Sennerby L, et al. Back-scattered electron imaging and elemental microanalysis of retrieved bone tissue following maxillary sinus floor augmentation with calcium sulphate. Clin Oral Implants Res. 2008;19: 814–822. DOI: 10.1111/j.1600-0501.2008.01550.x.

23. Smucker JD, Bobst JA, Petersen EB. B2A peptide on ceramic granules enhance posterolateral spinal fusion in rabbits compared with autograft. Spine. 2008; 33: 1324–1329.

24. Supova M. Problem of hydroxyapatite dispersion in polymer matrices: a review. J Mater Sci Mater Med. 2009; 20: 1201–1213.

25. Thalgott JS, Klezl Z, Timlin M, et al. Anterior lumbar interbody fusion with processed sea coral (coralline hydroxyapatite) as part of a circumferential fusion. Spine. 2002; 27: E518–E525.

26. Thalgott JS. Point of View. Spine. 2002; 27: E526.

27. Uchida A, Araki N, Shinto Y, et al. The use of calcium hydroxyapatite ceramic in bone tumour surgery. J Bone Joint Surg Br. 1990; 72: 298–302.

28. Vago R. Beyond the skeleton. Cnidarian biomaterials as bioactive extracellular microenvironments for tissue engineering. Organogenesis. 2008; 4: 18–22.

29. Van Haastert RM, Grote JJ, Van Blitterswljk CA, et al. Osteoinduction within PEO/PBT copolymer implants in cranial defects using demineralized bone matrix. J Mater Sci Mater Med. 1994; 5: 764–769.

30. Wigfield CC, Nelson RJ. Nonautologous interbody fusion materials in cervical spine surgery: how strong is the evidence to justify their use? Spine. 2001; 26: 687–694.

31. Younger EM, Chapman MW. Morbidity at bone graft donor sites. J Orthop Trauma. 1989; 3: 192–195.

32. Zarb G, Albrektsson T. Osseointegration – a requiem for the periodontal ligament? Editorial. Int J Periodont Res Dent. 1991; 11: 88–91.