Experimental substantiation of osteotransplant application in traumatic vertebral defects
https://doi.org/10.14531/2018.4.41-51
Abstract
Objective. To analyze the features of bone tissue formation during plasty of vertebral body defect or fracture with an allogeneic bone graft in an experiment in vitro.
Material and Methods. Models of the vertebral body defect (fracture of the cranioventral part with penetration into the nucleus pulposus) were created in an experiment on 20 mini-pigs of the same age. Plasty of traumatic defects was performed with allogeneic bone graft or autologous bone. CT, histological, and spectrometric studies of microscopic specimens were carried out at 14, 30, 90, and 180 day. Reparative osteogenesis, X-ray density, Ca and P content, and microhardness were studied.
Results. After implantation of allogeneic bone graft, an organ-specific bone similar to the recipient’s bone in morphological structure, X-ray density, mineral composition and microhardness, was formed on the 90th day (P = 0.01). After transplantation of autobone, the regenerate formed by this day in the central part was in a phase of resorption and restructuring with lower indices of X-ray density, content of Ca and P, and microhardness (P = 0.01).
Conclusion. Аfter plasty of vertebral body traumatic defects with allogeneic bone graft, the organ-specific bone tissue is formed at an earlier time and reliably exhibits greater mineralization and strength.
About the Authors
V. V. Rerikh
Novosibirsk Research Institute of Traumatology and Orthopaedics n.a. Ya.L. Tsivyan; Novosibirsk State Medical University
Russian Federation
Russian Federation
DMSc, Head of the Functional group, senior researcher
Frunze str.,17, Novosibirsk, 630091
professor of traumatology and orthopedics
Krasny pr., 52, Novosibirsk, 630091
Yu. A. Predein
Novosibirsk Research Institute of Traumatology and Orthopaedics n.a. Ya.L. Tsivyan
Russian Federation
Russian Federation
orthopedic traumatologist
Frunze str.,17, Novosibirsk, 630091
A. M. Zaidman
Novosibirsk Research Institute of Traumatology and Orthopaedics n.a. Ya.L. Tsivyan
Russian Federation
Russian Federation
DMSc, Prof., Head of the Functional group of Pathomorphology and Theoretical Research in Vertebrology in the Laboratory-Experimental Department
Frunze str.,17, Novosibirsk, 630091
A. D. Lastevsky
Novosibirsk Research Institute of Traumatology and Orthopaedics n.a. Ya.L. Tsivyan
Russian Federation
Russian Federation
orthopedic traumatologist, researcher
Frunze str.,17, Novosibirsk, 630091
V.A. Bataev V.A. Bataev V.A. Bataev
Novosibirsk State Technical University
Russian Federation
Russian Federation
DSc in Technics, Prof, Head of the Department of Material Science in Mechanical Engineering
K. Marksa pr., 20, Novosibirsk, 630073
A. A. Nikulina
Novosibirsk State Technical University
Russian Federation
Russian Federation
PhD in Technics, researcher of the Department of the Material Science in Mechanical Engineering
K. Marksa pr., 20, Novosibirsk, 630073
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Review
For citations:
Rerikh V.V., Predein Yu.A., Zaidman A.M., Lastevsky A.D., V.A. Bataev V.V., Nikulina A.A. Experimental substantiation of osteotransplant application in traumatic vertebral defects. Russian Journal of Spine Surgery (Khirurgiya Pozvonochnika). 2018;15(4):41-51. https://doi.org/10.14531/2018.4.41-51
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