LASER-INDUCED FLUORESCENCE FOR OSTEOPOROSIS DIAGNOSIS IN THORACIC AND LUMBAR VERTEBRAL FRACTURES

Objective - to investigate spectral characteristics of laserinduced fluorescence (LIF) of bone substances, and to develop a new technique of osteoporosis diagnosis. Sixty-nine patients surgically treated for thoracic and lumbar spine fractures were examined. Clinical examinations, plain radiography in two projections, CT and MRI were used for spine fracture diagnosis. Saville index was a criterion for osteopenia. Bone mineral density was assessed by dual-energy X-ray densitometry. Histological and histomorphometric analyses were applied to 46 fragments of vertebral bodies and spinous processes taken intraoperatively, and 96 autopsy fragments of vertebral bone, spinous processes, iliac crest, and the ninth rib taken from patients died of trauma who had no metabolic bone diseases in their previous history. Fluorescence spectra were analyzed with multichannel system of spectrum registration. Gas-discharge KrF excimer laser with a wave length of λ = 28 nm and impulse energy of 5-10 mJ was used as a source of radiation. Spectral characteristics of fluorescence intensity of biological substances forming a bone tissue have shown that the most expressed fluorescence of hydroxyapatite is observed in the band of 380-450 nm. The LIF technique permits not only to differentiate the structural-functional layers of bone, but also to determine a bone tissue mineralization degree. The possibility to perform express diagnosis is one of the advantages of the technique. It is especially topical for traumatology.

osteoporosis, densitometry, laser-induced fluorescence

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